urinary aminopeptidase activities as early and predictive biomarkers of renal dysfunction in cisplatin-treated rats尿氨肽酶的活动早和预测生物标志物cisplatin-treated老鼠的肾脏功能障碍.pdf

Urinary Aminopeptidase Activities as Early and Predictive Biomarkers of Renal Dysfunction in Cisplatin- Treated Rats ´ 1 ´ 2 ´ 1 3 ´ Andres Quesada , Felix Vargas , Sebastian Montoro-Molina , Francisco O’Valle , Marıa ´ ´ 3 4 1 ´ 1 Dolores Rodrıguez-Martınez , Antonio Osuna , Isabel Prieto , Manuel Ramırez , Rosemary Wangensteen1* ´ ´ ´ ´ ´ 1 Area de Fisiologıa, Departamento de Ciencias de la Salud, Universidad de Jaen, Jaen, Spain, 2 Departamento de Fisiologıa, Facultad de Medicina, Universidad de ´ ´ ´ Granada, Granada, Spain, 3 Departamento de Anatomıa Patologica, Facultad de Medicina, Universidad de Granada, IBIMER Granada, Spain, 4 Servicio de Nefrologıa, Hospital Virgen de las Nieves, Granada, Spain Abstract This study analyzes the fluorimetric determination of alanyl- (Ala), glutamyl- (Glu), leucyl-cystinyl- (Cys) and aspartyl- aminopeptidase (AspAp) urinary enzymatic activities as early and predictive biomarkers of renal dysfunction in cisplatin- treated rats. Male Wistar rats (n = 8 each group) received a single subcutaneous injection of either saline or cisplatin 3.5 or 7 mg/kg, and urine samples were taken at 0, 1, 2, 3 and 14 days afte