comparative dynamics of retrograde actin flow and focal adhesions formation of nascent adhesions triggers transition from fast to slow flow比较逆行肌动蛋白动力学流和焦粘连形成新生粘连引发从快速过渡到缓慢流动.pdf

Comparative Dynamics of Retrograde Actin Flow and Focal Adhesions: Formation of Nascent Adhesions Triggers Transition from Fast to Slow Flow 1,2 3 ´ 1,5 2,4 Antonina Y. Alexandrova , Katya Arnold , Sebastien Schaub , Jury M. Vasiliev , Jean-Jacques 1 3 1 Meister , Alexander D. Bershadsky *, Alexander B. Verkhovsky * ´ ´ 1 Ecole Polytechnique Federale de Lausanne, Laboratory of Cell Biophysics, Lausanne, Switzerland, 2 Belozersky Institute of Physical and Chemical Biology, Moscow State University, Moscow, Russia, 3 Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel, 4 Cancer Research Center, Russian Academy of ´ Medical Sciences, Moscow, Russia, 5 Institut ‘‘Biologie du Developpement et Cancer’’ UMR 6543–CNRS, Nice, France Abstract Dynamic actin network at the leading edge of the cell is linked to the extracellular matrix through focal adhesions (FAs), and at the same time it undergoes retrograde flow with different dynamics in two distinct zones: the lamellipodium (peripheral zone of fast flow), and the lamellum (zone of slow flow located between the lamellipodium and the cell body). Cell migration involves expansion of both the lamellipodium and the lamellum, as well as formation of new FAs, but it is largely unknown how the position of the boundary between the two flow zones is defined, and how FAs and actin flow mutually influence each other. We investigated dynamic relationship between focal adhesions and the boundary between the two flow zones in spreading cells. Nascent FAs first appeared in t