利用简单重复序列分子标记分析探究我国侧耳栽培菌株的遗传多样性.pdf

ACTAEDULISFUNGI@2012．19(2)：15～25 of18 CharacterizationNovel SSR Polymorphic MarkersandEvaluationofGenetic Within Diversity MushroomStrainsCultivatedinChina Oyster WU Dandanl～，ZHANGZhenl，ZHANG Dandan3，GU Ruiying孙，HU Jingan92， HU Xuemei2 Qingxiu2，ZU0 (1SchoolofLifeScience，Anhui 230036，China；2 AgriculturalUniversity，Hefei，AnhuiKeyLaboratory ofMicrobialResourcesCollectionand of of Preservation，MinistryAgriculture，InstituteAgricultural Resourcesand of 100081，China； RegionalPlanning，ChineseAcademyAgriculturalSciences，Beijing 3 ofScienceand 100089，China) BeijingAcademy Technology，Beijing oneof cultivated mushrooms themost of edible Abstract：Oysterrepresent importantgroupscommercially mushroomsworldwide．Inthis novel were study，18polymorphicsimplesequencerepeat(SSR)markers andcharacterizedfromPleurotusostreatus，strainACCC50838．7lhenumberofallelesdetected developed foreachSSRlocusvariedbetweenthreeandtwelveacrossthe52 mushroomcultivars，48ofwhich oyster were asP．ostreatusandfouras information previouslydesignated P．pulmonarius．Thepolymorphism theSSRs from0．34to0．84．All52cultivarsweredifferentiatedthe18SSR content(P1C)of