the effect of surface nanometre-scale morphology on protein adsorptionnanometre-scale表面形态对蛋白质吸附的影响.pdf

The Effect of Surface Nanometre-Scale Morphology on Protein Adsorption 1,2 1,2 1 1,3 Pasquale Emanuele Scopelliti , Antonio Borgonovo , Marco Indrieri , Luca Giorgetti , Gero 2 4 ` 1 1,2 Bongiorno , Roberta Carbone , Alessandro Podesta , Paolo Milani * ` 1 Interdisciplinary Centre for Nanostructured Materials and Interfaces (CIMaINa) and Physics Department, Universita degli studi di Milano, Milan, Italy, 2 Micro and Nano Fabrication Platform, Fondazione Filarete, Milan, Italy, 3 Department of Experimental Oncology, European Institute of Oncology Campus IFOM-IEO, Milan, Italy, 4 Tethis srl, Milan, Italy Abstract Background: Protein adsorption is the first of a complex series of events that regulates many phenomena at the nano-bio interface, e.g. cell adhesion and differentiation, in vivo inflammatory responses and protein crystallization. A quantitative understanding of how nanoscale morphology influences protein adsorption is strategic for providing insight into all of these processes, however this understanding has been lacking until now. Methodology/Principal Findings: Here we introduce novel methods for quantitative high-throughput characterization of protein-surface interaction and we apply them in an integrated experimental strategy, to study the adsorption of a panel of proteins on nanostructured surfaces. We show that the increase of nanoscale roughness (from 15 nm to 30 nm) induces a decrease of protein binding aff