the immunomodulatory activity of meningococcal lipoprotein ag473 depends on the conformation made up of the lipid and protein moieties脑膜炎球菌的免疫调节活动脂蛋白ag473取决于脂质和蛋白质的构象由半个.pdf

The Immunomodulatory Activity of Meningococcal Lipoprotein Ag473 Depends on the Conformation Made up of the Lipid and Protein Moieties 1 2 2 3 4,5 3 Ching-Liang Chu , Yen-Ling Yu , Yueh-Chen Kung , Pei-Yu Liao , Ko-Jiunn Liu , Yen-Tzu Tseng , Yuan- 3 2 2 2,3 Chuen Lin , Steve Shih-Yang Hsieh , Pele Choi-Sing Chong , Chiou-Ying Yang * 1 Graduate Institute of Immunology, College of Medicine, National Taiwan University, Taipei, Taiwan, 2 Vaccine Research and Development Center, National Health Research Institutes, Miaoli, Taiwan, 3 Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan, 4 National Institute of Cancer Research, National Health Research Institutes, Tainan, Taiwan, 5 School of Medical Laboratory Science and Biotechnology, Taipei Medical University, Taipei, Taiwan Abstract We have previously demonstrated that the meningococcal antigen Ag473 in the presence of Freund’s adjuvant can elicit protective immune responses in mouse challenge model. In this study, we evaluated the structural requirement for the immunological activity and the possible signaling pathway of recombinant Ag473 antigen produced in E. coli. We found that lipidated Ag473 (L-Ag473) possesses an intrinsic adjuvant activity that could be attributed to its ability to activate dendritic cells and promote their maturation. In addition, we found that L-Ag473 can activate human monocytes and promote maturation of human monocyte-derived dendritic cells. These results provide an indirect support that L-Ag473