水稻结实期不同粒位籽粒相关内源激素含量和关键酶活性的差异及其.DOC

水稻结实期不同粒位籽粒相关内源激素含量 和关键酶活性的差异及其与品质的关系 董明辉1，赵步洪2，吴翔宙1，陈 涛1，杨建昌1 （1扬州大学农学院/江苏省作物遗传生理国家重点实验室培育基地，江苏扬州 225009；2扬州里下河地区农业科学研究所，江苏扬州 225007） 摘要：【目的】研究灌浆期水稻穗上不同粒位籽粒内源激素含量和关键酶活性的差异及其与籽粒品质形成的关系。【方法】以中熟籼稻扬稻6号和中熟粳稻扬粳9538为材料，按照一次枝梗数将稻穗分为上、中、下三个部位，以穗中部一、二次枝梗不同粒位籽粒为研究对象。【结果】灌浆前期开花较早的籽粒中玉米素（Z）+玉米素核苷（ZR）、吲哚乙酸（IAA）、脱落酸(ABA)含量以及淀粉合成酶（SSase）、淀粉分支酶（Q酶）活性明显高于同枝梗上开花较迟的籽粒，灌浆后期则相反；籽粒开花越早，其内源激素含量以及酶活性到达最大值的时间越早，峰值也越高。灌浆前期ABA、Z+ZR、IAA含量、ABA/（Z+ZR+IAA）比值以及SSase酶和Q-酶活性与千粒重、直链淀粉累积速率、直链淀粉含量呈显著或极显著正相关，与垩白度呈显著或极显著负相关，灌浆后期则相反；灌浆前期SSase和Q-酶活性与胶稠度呈负相关，中、后期则相反；与粗蛋白含量的相关两品种存在差异，与整精米率相关不显著。【结论】灌浆期水稻穗上不同粒位籽粒内源激素含量与关键酶活性存在差异；不同粒位间籽粒米质差异的形成与籽粒内源激素含量与关键酶活性密切相关。 关键词：水稻；粒位；激素；酶活性；品质 Difference in Hormonal Content and Activities of Key Enzymes in the Grains at Different Positions on a Rice Panicle During Grain Filling and Their Correlations with Rice Qualities DONG Ming-hui, ZHAO Bu-hong, WU Xiang-zhou, Chen Tao, YANG Jian-chang (1College of Agronomy/Fostering Base for the National Key Laboratory of Crop Genetics and Physiology, Jiangsu Province, Yangzhou University, Yangzhou 225009, Jiangsu; 2Lixiahe Region Agricultural Research Institute of Jiangsu, Jiangsu Province, Yangzhou 225007) Abstract: 【Objective】This study investigated the differences of hormonal content and activities of key enzymes in the grains at different positions on a rice panicle during grain filling and their correlation with rice quality. 【Method】 Two rice cultivars, Yangdao 6 (indica) and Yangjing 9538 (japonica), were grown. All primary branches on a panicle were evenly divided into three parts of the upper, middle and base. The grains were numbered as 1 to 6 on a primary branch and 1 to 3 or 4 on a secondary branch from the top to base. The grains at the middle part of a panicle were measured. 【Result】 The contents of zeatin (Z) + zeatin riboside (ZR), indole -3- acetic acid (IAA) and abscisic acid (ABA) and activities of starch synthase (SSase), starch branching enzyme (Q-enzyme) in earlier flowered grains were obviousl