人参皂苷Rg1对大鼠急性心肌缺血抗氧化损伤-国家心血管病中心.PDF

164 中国循环杂志 2015 年 2 月 第 30 卷 第 2 期（总第 200 期）Chinese Circulation Journal，February，2015，Vol. 30 No.2（Serial No.200） 基础与实验研究 人参皂苷 Rg1 对大鼠急性心肌缺血抗氧化损伤指标及超微结构的影响 * 张庆勇 ，陈燕萍，刘芬，陈霞 摘要 目的 : 研究人参皂苷 Rg1 对大鼠急性心肌缺血抗氧化损伤指标及超微结构的影响。 方法 : 建立大鼠急性心肌缺血模型，大鼠随机分为假手术组、急性心肌缺血模型组（模型组）、人参皂苷 Rg1 5 mg/kg 组和人参皂苷 Rg1 10 mg/kg 组，腹腔注射给药两周后取材。氯化三苯基四氮唑（TTC）法测量心肌梗死面积，电子显微镜观 察心肌组织超微结构变化，比色法测定大鼠血清超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性，以及丙 二醛（MDA）含量。 结果 : 与模型组比较，人参皂苷 Rg1 5mg/kg 组和人参皂苷 Rg1 10 mg/kg 组可明显减少心肌梗死面积，减少心肌 组织损伤，人参皂苷 Rg1 10 mg/kg 可明显提高大鼠血清 SOD 和 GSH-Px 活性，降低丙二醛的含量。 结论 : 人参皂苷 Rg1 对急性心肌缺血具有保护作用，抗氧化损伤作用是其保护作用机制之一。 关键词 人参皂苷 Rg1；心肌缺血；抗氧化损伤指标；超微结构 Effect of Ginsenosides-Rg1 on Acute Myocardial Ischemic Anti-oxidative Injury Indexes and Ultra- structure in Experimental Rats ** ZHANG Qing-yong , CHEN Yan-ping, LIU Fen, CHEN Xia. Department of Pharmacology, College of Basic Medical Sciences, Jilin University, Changchun (130021), Jilin, China Corresponding Author: CHEN Xia, Email: chenx@ Abstract Objective: To study the effect of ginsenosides-Rg1 on acute myocardial ischemic anti-oxidative injury indicators and ultra-structure in experimental rats. Methods: The acute ischemia model was established in experimental rats and the animals were randomly divided into 4 groups: Sham operation group, Model group, Ginsenoside-Rg1 5 mg/kg group and Ginsenoside-Rg1 10 mg/kg group. The rats were treated by intraperitoneal injection for 2 weeks. The area of myocardial infarction was measured by 2, 3, 5-triphenyltetrazolium chloride (TTC) staining, the structural change of myocardial tissue was observed by electron microscope. The activities of blood superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) content were exa