小鼠脂肪源间充质干细胞在重型再生障碍性贫血中的应用.pdf

碟 基础医学 中国组织工程研究与临床康复第2卷第钳期2008—10—21出版 JournalofClinicalRehabilitativeTissueEngineeringResearch October21．2008 Vo1．12No．43 小鼠脂肪源间充质干细胞在重型再生障碍性贫血中的应用术术★ 王 萍 。，房佰俊 ，宋永平 ，董子明 Applicationofadiposetissue-derivedmesenchymalstem cellsinsevereaplasticanemiamice WangPing’l_FangBai-jun’，SongYong—ping’，DongZi—ming ， Abstract De~ ntof BACKGROUND：Bonemarrow mesenchymalstem eellandhematopoieticstem eelltransplantationisoneofmethod ssolving Hematology,Henan TumorHospital， hematopoieticreestablishment．Howeverthemehtodofextractingbonemarrow forisolationandamplificationmesenchymalstem HenanInstituteof eellscannotbeacceptedbymosthealthYdonorsinclinic． Hematology, OBJECTIVE：Toexploreeffectsofmouseadiposetissue．derivedmesenchymalstemcellsonhematopoieticfunctionofallogeneic Zhengzhou 450008 HenanProvince， severeaplasticanemiamice． China； el~lmentof DESIGN。TIMEAND SETTING：ThecytologyinvivoexperimentwasperformedathteCenrtalLaboratory，HenanTumorHospital Pathologyan(I 丘om M aytoNovember2007． Physiology,Basic MedicalCollege， MATERIALS：TwentymaleBALB，c(H一2Ka)miceaged6 8weeks，andfiftyfemaleC57BL，6(H一2K。)miceaged78weekswere ZhengzhouUniversity, purchasedfrom HenanProvincialAnimalExperimentalCenter． Zhengzhou 450052， M ETH0DS：Adiposetissue—derivedmesenchymalstem cellsfrom 20maleBALEI／cmiceweredissociatedandculturedbythe HenanProvince，China adherencemethod．Bonemarrow eellsrfom 20femaleC57BI26fH一2K。)micewereblownandbeaten．Erythrocyteswereremoved WangPing★ afterincubatinginEDTA—NH4C1method．Theremainingwasbonemarrow karyocytes．Theremaining30femaleC57BL，6(H．2K ) SutdyingforE~aster’s micereceivedagenera1irradiationwith3．0GyOOCo-Y． At4．5and6daysafterirradiation．ratsweresubcutane