Fundamentals of HPLC - USTC（高效液相色谱基础中国科技大学）.pdf
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HPLC Basics Fundamentals of Liquid
Chromatography (HPLC)
Courtesy of Agilent Technologies, Inc.
Detector
Compound B
Control and
Compound A
Column and data processing
Compound C
column oven
0 2 4 6 8 10 12 14
min
Pump Injector
Chromatogram
HPLC Basics
Page 1
Fundamentals of High Performance Liquid
Chromatography (HPLC)
This course will enable you to:
• Explain the general principles of HPLC analyses
• Know the major application areas of HPLC
• Identify the major components of an HPLC system
and explain their principles of operation
HPLC Basics
Page 2
Chromatographic Separation Techniques
Which sep
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