山桐子果实成熟度对种子萌发与果实油品质的影响以及干旱胁迫下不同种源山桐子的生理生化研究.pptx

山桐子果实成熟度对种子萌发与果实油品质的影响以及干旱胁迫下不同种源山桐子的生理生化研究 叶片含水量的测定/相对外渗率的测定/ 叶绿素含量的测定/叶片荧光参数的测定 生理指标：丙二醛（MDA）和酶（SOD,POD,APX)活的测定 、脯氨酸和可溶性糖的测定 Identification of candidate genes for drought tolerance in coffee by high-throughput sequencing in the shoot apex of different Coffea arabica cultivars (IAPAR59, drought-tolerant and Rubi, drought-susceptible) of Coffea arabica grown in the field under control(irrigation) and drought conditions using the pyrosequencing of RNA extracted from shoot apices and analysing the expression of 38 candidate genes. methods: field experiment----sample(leaf/plagiotropic buds for qpcr expression analysis/ meristem and leaf primoridium for pyrosequencing-----RNA isolation, DNA synthesis and 454-sequencing-----Transcriptome assembly and automatic annotation/Digital gene expression analysis---- --Functional annotation of differentially expressed genes-----selection of candidate gene(45 ）-----expression profiles of candidate genes--- Monitoring drought under field conditions castk1 genes with reduced expression under drought conditions