yeast mitochondrial biogenesis a role for the puf rna-binding protein puf3p in mrna localization酵母线粒体生物起源的角色puf rna结合蛋白puf3p mrna本地化.pdf

Yeast Mitochondrial Biogenesis: A Role for the PUF RNA- Binding Protein Puf3p in mRNA Localization 1. 1. 1 2 2 Yann Saint-Georges , Mathilde Garcia , Thierry Delaveau , Laurent Jourdren , Stephane Le Crom , 2 2 1 1,2 Sophie Lemoine , Veronique Tanty , Frederic Devaux *, Claude Jacq * ´ ´ ´ ´ ´ 1 Laboratoire de Genetique Moleculaire CNRS-UMR8541, Ecole Normale Superieure, Paris, France, 2 Plateforme Transcriptome IFR36, Ecole Normale Superieure, Paris, France Abstract The asymmetric localization of mRNA plays an important role in coordinating posttranscriptional events in eukaryotic cells. We investigated the peripheral mitochondrial localization of nuclear-encoded mRNAs (MLR) in various conditions in which the mRNA binding protein context and the translation efficiency were altered. We identified Puf3p, a Pumilio family RNA- binding protein, as the first trans-acting factor controlling the MLR phenomenon. This allowed the characterization of two classes of genes whose mRNAs are translated to the vicinity of mitochondria. Class I mRNAs (256 genes) have a Puf3p binding motif in their 3’UTR region and many of them have their MLR properties deeply affected by PUF3 deletion. Conversely, mutations in the Puf3p binding motif alter the mitochondrial localization of BCS1 mRNA. Class II mRNAs (224 genes) have no Puf3p binding site and their asymmetric localization is not affected by the abs