damaged dna binding protein 2 plays a role in breast cancer cell growth受损的dna结合蛋白2在乳腺癌细胞生长过程中发挥作用.pdf

Damaged DNA Binding Protein 2 Plays a Role in Breast Cancer Cell Growth 1 2 1 3 ` 3 3 Zilal Kattan , Sophie Marchal , Emilie Brunner , Carole Ramacci , Agnes Leroux , Jean Louis Merlin , 1 1 1 Lionel Domenjoud , Michel Dauc¸a , Philippe Becuwe * ´ ´ ´ ´ ` 1 Laboratoire de Biologie Cellulaire du Developpement, EA 3446 Universite Henri Poincare-Nancy Universite, Vandoeuvre-les-Nancy, France, 2 Centre Alexis Vautrin, UMR ´ ´ ´ ´ 7039 Institut Polytechnique de Lorraine/Universite Henri Poincare-Nancy Universite/ CNRS, Vandoeuvre les Nancy, France, 3 Unite de Biologie des Tumeurs du Centre ´ ` Alexis Vautrin, EA3452 Nancy Universite, Vandoeuvre les Nancy, France Abstract The Damaged DNA binding protein 2 (DDB2), is involved in nucleotide excision repair as well as in other biological processes in normal cells, including transcription and cell cycle regulation. Loss of DDB2 function may be related to tumor susceptibility. However, hypothesis of this study was that DDB2 could play a role in breast cancer cell growth, resulting in its well known interaction with the proliferative marker E2F1 in breast neoplasia. DDB2 gene was overexpressed in estrogen receptor (ER)-positive (MCF-7 and T47D), but not in ER-negative breast cancer (MDA-MB231 and SKBR3) or normal mammary epithelial cell lines. In addition, DDB2 expr