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响应面法优化桑叶多糖的超声波辅助萃取工艺.pdf

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2016年 2月 食品研究与开发 第37卷第3期 FoodResearchAndDevelopment 工艺技术 —=== 116 DOI:10.3969~.issn.1005-6521.2016.03.030 响应面法优化桑叶多糖的超声波辅助萃取工艺 张嫱l1。熊祥 (1.北方民族大学生命科学与工程学院,宁夏银川 750021;2.合肥工业大学 生物与食品学院, 安徽 合肥230009) 摘 要:利用桑树叶片为原料,运用响应面法对桑叶多糖的超声波辅助提取工艺进行优化研究。根据BOX (Box— Benhnken)试验设计原理,确定超声时间、超声温度、萃取液固比对桑叶多糖提取率的影响。结果表明:超声波辅助萃取 桑叶多糖的最佳工艺条件为超声温度 52℃,超声时间36rain,液固比40:l(mLg/),在此萃取条件下,多糖的萃取率可达 3.2l%。 关键词 :桑叶;多糖;超声辅助提取;响应面分析 OptimizationofUltrasound-assistedExtractionofPolysaccharidefrom M ulberryLeavesby ResponseSurfaceMethodology ZHANGQiang_|.XIONGXiang (1.CollegeofBiologicalScienceandEngineering,BeifangUniversityofNationalities,Yinchuan750021, Ningxia,China;2.CollegeofBiotechnologyandFoodEngineering,HefeiUniversityofTechnology,Hefei 230o09,Anhui,China) Abstract:Technologicalconditionsofextractionofmulberryleaveswithultrasound——assistedextractionwere optimizedfordevelopingandutilizingtheresourceof’mulberryleaves,byresponsesurfacemethodologyasthe methodofstudyingpolysaccharideofmulberryleaves.AccordingtotheprincipleofBox-Behnkencentral compositedesign,uhrasonictime,extractiontemperatureandsolid—liquidratiowere chosenasresponse factors,extractionofPolysaccharidefrommulberryleaves (MLP)massasresponsevalue,andathree—factor andthreelevelscentralcompositedesignwasadopted todeterminethe influenceofvarioustechnological conditions.Theresultsshowedthatbetterextractionconditionswere52℃ oftemperature,36minofperiod, 40:1(mug)ofliquid—solidratio.Undertheseconditions,theyieldofMLPwas3.21%. Keywords:mulberryleaves;polysaccharide;ultrasound-assistedextraction;responsesurfacemethodoloyg 作者简介:张嫱(1980一),女(汉),讲师,博士,研究方向:天然
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