assays to detect β-tubulin codon 200 polymorphism in trichuris trichiura and ascaris lumbricoides鞭虫化验检测β-tubulin 200密码子多态性trichiura和蛔虫.pdf

Assays to Detect b-Tubulin Codon 200 Polymorphism in Trichuris trichiura and Ascaris lumbricoides 1 2 2{ 3 4 Aissatou Diawara , Lesley J. Drake , Richard R. Suswillo , Jimmy Kihara , Donald A. P. Bundy , 1 1 5 1 Marilyn E. Scott , Carli Halpenny , J. Russell Stothard , Roger K. Prichard * 1 Institute of Parasitology, McGill University, Ste. Anne de Bellevue, Quebec, Canada, 2 The Partnership for Child Development, Imperial College School of Medicine, London, United Kingdom, 3 Kenya Medical Research Institute, Nairobi, Kenya, 4 World Bank, Washington D.C., United States of America, 5 Natural History Museum, London, United Kingdom Abstract Background: The soil-transmitted helminths (STH) Ascaris lumbricoides and Trichuris trichiura are gastrointestinal parasites causing many disabilities to humans, particularly children. The benzimidazole (BZ) drugs, albendazole (ALB) and mebendazole (MBZ), are commonly used for mass treatment for STH. Unfortunately, there is concern that increased use of anthelmintics could select for resistant populations of these human parasites. In veterinary parasites, and lately in filarial nematodes, a single amino acid substitution from phenylalanine to tyrosine, known to be associated with benzimidazole resistance, has been found in parasite b-tubulin at position 200. We have developed pyrosequencer assays for codon 200 (TTC or TAC) in A. lumbricoides and T. trichiura to screen for this single nucleotide polymorphism (SNP). Method and Findings: Pyrosequencing assays were developed and evaluated for detecting the TTC or T