天冬氨酸激酶代谢调控的研究进展-食品科学.PDF

270 2016, Vol.37, No.07 食品科学 ※专题论述 天冬氨酸激酶代谢调控的研究进展 杨宇亭，闵伟红* （吉林农业大学食品科学与工程学院，小麦和玉米深加工国家工程实验室，吉林 长春 130118 ） 摘  要：在微生物发酵合成天冬氨酸族氨基酸的代谢途径中，天冬氨酸激酶（aspartokinase，AK ）是限制碳源和氮 源流速的限速酶，是决定能否获得高产天冬氨酸族氨基酸的关键之一。本文介绍了AK 的重要性、不同来源AK 的整 体结构、效应结合位点的特点和调控机制研究进展，并对AK代谢调控的发展做出展望，为进一步明确AK代谢调控 机制，促进天冬氨酸族氨基酸产业化发展提供参考和依据。 关键词：天冬氨酸激酶；合成途径；效应结合位点；调控机制 Review of Research on Metabolic Regulation of Aspartokinase * YANG Yuting, MIN Weihong (National Engineering Laboratory on Wheat and Corn Further Processing, College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China) Abstract: Aspartate kinase (aspartokinase, AK) is a rate-limiting enzyme controlling the carbon and nitrogen flux into the biosynthesis pathways of amino acids of the aspartic acid family in microorganisms. It is a key enzyme to determine the ability to obtain aromatic aspartic amino acids. This article describes recent progress in understanding the importance of aspartatekinase, the general structure of aspartatekinases from different species, features of their binding sites and their regulatory mechanisms. Moreover, future research trends in this area are foreseen. The aim of this review is to provide further understanding of the mechanism of metabolic regulation of aspartatekinase and accordingly promote the industrial development of aspartic acid family amino acids. Key words: aspartatekinase; biosynthetic pathway; binding sites; regulatory mechanisms DOI:10.7506/spkx1002-6630-201607048 中图分类号：Q71 文献标志码：A 文章编号：1002-6630 （2016 ）07-0270-06 引文格式： 杨宇亭