基于代谢组学的细胞内源性代谢物研究进展.pdf

978 药学学报 Acta Pharmaceutica Sinica 20 12, 47 (8): 978 985 于代谢组学的细胞内源性代谢物研究进展 张辰辰, 孙立新* (沈阳药科大学药学院, 辽宁 沈阳 1106 11) 摘要: 代谢组学是继基因组学、转录组学和蛋白质组学之后新兴的一种定性和定量分析复杂生物样品中所 有小分子代谢物的组学方法, 也是目前组学研究领域的热点之一。代谢组学主要考察生物体系受刺激或扰动后 内源性代谢物的变化, 是系统生物学的有机组成部分。近年来在代谢组学研究中, 细胞内源性代谢物的研究已取 得了很大进展。本文结 代谢组学的基本含义, 综述了细胞样品前处理方法及代谢靶向分析、代谢轮廓分析、 代谢组学分析和代谢足迹分析在细胞方面的研究进展。 关键词: 代谢组学; 内源性代谢物; 细胞样品前处理; 代谢靶标分析; 代谢轮廓分析; 代谢组学分析; 代谢 足迹分析 中图分类号: R9 17 文献标识码: A 文章编号: 05 13-4870 (20 12) 08-0978-08 Research progress on intracellular metabolites based on metabolomics * ZHANG Chen-chen, SUN Li-xin (School of Pharmacy, Sheny ang Pharmaceutical University, Sheny ang 110611, China) Abstract: Being an essential component of systematic biology, metabolomics has received attention in recent years. It is a post genomic technology aimed at qualitative and quantitative analysis of all low molecular- mass metabolites present in complex biological samples, and mainly investigates the change of endogenous metabolites of a stimulated or disturbed biological system. Investigations into intracellular endogenous metabolites in metabolomics have great advancement in recent years. This review outlines the progress of metabolomics in cell culture analysis including sample preprocessing methods and metabolite target analysis, metabolic profiling analysis, metabolomics analysis and metabolic footprinting analysis. Key words: metabolomics; endogenous metabolite; cell sample preprocessing; metabolite target analysis; metabolic profiling analysis; meta