hplc法测定玫瑰花瓣中槲皮素含量_艾合买提_买买提.pdf

· · 　　228 　2010, Vo.l34, No.5　 　2010年第34卷　第5期　第　　228页　 　 ProgressinPharmaceuticalSciences　 do:i10.3969/.jissn.100 1-5094.20 10.05.007 HPLC法测定玫瑰花瓣中槲皮素含量 艾合买提·买买提, 　买合布白·阿不都热依木, 　艾克白尔·买买提, 阿布都热依木·阿布都克日木＊ (新疆维吾尔自治区维吾尔医医院科研科, 新疆 乌鲁木齐 830049 []　: HPLC, 。: -1 :SymmetryShieldRP18(150 mm×4.6 mm, 5 μm;:25 ℃;:368 nm;:1.0 mL·min;: -0.4% (60 ∶40 ;:10 μL。: 98.4%(RSD=1.7 8%, n=9 ; -1 (3.05 ±0.090 mg·g (RSD=2.95%, n=3 , (0.405 ±0.013 -1 -1 mg·g(RSD=3.21%, n=3 ;(1.841±0.013 mg·g (RSD=1.84%, -1 n=3 , (0.225 ±0.007 mg·g (RSD=3.11%, n=3 , 。:、、, 。 []　HPLC;;; []　O657.7 2　　[]　B　　[] 1001-5094 (2010 05-0228-03 DeterminationofQuercetininRosarugosaPetalbyHPLC AhmatMama,t　MahbubaAbdureymi, 　AkbarMama,t　AbdureymiAbdukermi (ResearchDepartmen,tUyghurMedicineHospitalofXinjiangUyghurAutonomousRegion, Urmqi830049, China [Abstract]　Objective:ToestablishaHPLCmethodforthedeterminationofquercetininRosarugosa petalandtocomparethecontentsofquercetininRosarugosafromtwosources.Methods:Thedetermina- tionwasperformedonaSymmetryShieldRP18 (150 mm×4.6 mm, 5 μm, andmethanol-0.4% phos- phoricacid(60 ∶40 wereusedasmobilephaseatacolumntemperatureof25 ℃, atdetectionwave- -1 lengthof368 nm, andwithaflowrateof1.0 mL min andaninjectionvolumeof10 μL.Results:The averagerecoveryofquercetinwas98.4%(RSD=1.