蛋白质复合物的纯化.pdf

Purification of 蛋白质复合物的纯化 Multiprotein Complexes Purification of multiprotein complexes 蛋白质复合物的纯化 Outline 内容 • Introduction • 简介 – 蛋白质-蛋白质相互作用 – Protein-protein Interaction – 纯化的困难 – Challenges in purification – 分析技术 – Analytical techniques • Purification • 纯化 – “ng to µg” scale – “ng to µg” 规模 – “µg to mg” scale – “µg to mg” 规模 • Summary • 结论 Introduction 简介 1 / GE / n o i t c 介 u d 简 o r t n Protein-protein interactions 蛋白质-蛋白质复合物 I • 5000 interactions have been mapped in C. elegans