好氧反硝化细菌的筛选鉴定及其反硝化反应条件优化_周莉.pdf

Acta Agriculturae Zhejiangensis, 2011, 23 (5 ):942 - 947 http:// www. zjnyxb. cn , , , . [J]. ,2011,23(5 ):942 -947. 1, 2 2 2 2 2 1, * 周　莉 , 汤江武 , 王　新, 姚晓红, 吴逸飞 , 葛向阳 1 2 ( , 430070; , 310021) 　:、, , 、、pH、、。, 35 GC5, 16S rDNA , (Pseudomonas sp. )。、15 ∶1, 1%, pH 7 .5, 160 r min- 1 30 ℃, , 99 .19% 53.83%。 :;;; + :Q 939 .11 2　　　　　:A :1004 - 1524 (2011 )05 -0942 - 06 Screening and identification of aerobic denitrifiers and the optimization of denitrification con- ditions 1,2 2 2 2 2 1, * ZHOU Li , ANG Jiang-wu ,WANG Xin ,YAO Xiao-hong , WU Yi-fei , GE Xiang-yang 1 2 ( College of Lif e Science and Technology, Huazhong Agricultural University, Wuhan 4 0070, China;Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 10021, China ) Abstract:Aerobic denitrifying bacteria were isolated directly from the aquaculture water, sludge and rural rivers. Following characterizing the phylogeny of the strains with the highest denitrifying capability, the effects of carbon source, C/ N ratio, initial pH, inoculation quantity, rotational speed and temperature on the characteristics of deni- trification were studied. he results showed that 35 strains of aerobic denitrifiers were obtained by primary screening on plates of Bromothymol Blue medium. One strain named GC5, which had the highest denitrifying capability was i- dentified after secondary screening and was designated as Pseudomonas sp. based on the analysis of 16S rDNA se- quence and phylogenetic analysis. Furthermore, the optimum conditions for denitrification