弓形虫与异尖线虫的免疫诊断和分子检测.ppt

* * * * * Potential implication for the development of schistosomiasis therapies by proteomic studies * DeMarco R, Verjovski-Almeida S: Schistosomes--proteomics studies for potential novel vaccines and drug targets. Drug Discov Today 2009, 14:472-478. * Proteomic analysis of Schistosoma japonicum against artesunate * In vivo In vitro Treatment group Dynamic unloading S. japonicum Culture of Schistosomula and adult Control Animal model Treatment group Control Confirmation the proteins 2DE profiles of proteins before and after artesunate treatment Analysis of 2DE profiles Identification of artesunate treatment-associated proteins Gene Ontology analysis Construct biological function network of potential proteins for the schistosomiasis therapies with artesunate. Pathway analysis * * Thanks for your attention! Good morning, Professor Ohta, professor Xia, Doctor Kumaga and every one here! Today, I want to talk some methods to detect Toxoplasma gondii, Schistosoma japonicum and Anisakis. * My talk contains three parts.And so, Let’scome in the first part: LAMP for early detection of T. gondii infection in mice. * There are many papers about LAMP reported in the Pubmed database. We can see the development trend of LAMP in parasites from this graph. * Total 65 LAMP related papers were reported in parasites study, there is 18.5 percent in plasmodium and 7.7 percent in Toxoplasma. To our delight, No reports about Anisakis detection by LAMP! * Approximately one third of world’s population is infected with T. gondii , and the infection rate is 12.3% in China. There are numbers of different assays to detect T. gondii, LAMP is more simple, sensitive and specific than these methods and no need for expensive equipment and long reaction time. * Based on B1 gene,we developed the LAMP at 65℃ for 1h and detected the mice blood samples at 1, 3 and 5 dpi . * The limit of detection for nested PCR was 1 ng to 100 picogram per microliter of T. gondii DNA template. Therefore, the