茶叶儿茶素的益生元活性研究.pdf

现代食品科技 Modern Food Science and Technology 2015, Vol.31, No.4 茶叶儿茶素的益生元活性研究 杨阳，张鑫，翁佩芳，吴祖芳 （宁波大学海洋学院，浙江宁波 315211） 摘要：近年来，茶叶儿茶素调节肠道微生态的效果受到广泛关注。本研究通过大孔树脂柱层析分离纯化，制备得到纯度大于 98% 的茶叶儿茶素 EGCG、EGCG3’’Me、ECG、ECG3’’Me。通过测定不同发酵时间点（0、6、12、24 h）体外培养中肠道菌群的变化以 及短链脂肪酸的含量，评价茶叶儿茶素调节肠道微生态的作用。茶叶儿茶素在体外厌氧发酵 24 h 时，对于肠道有益细菌（双歧???菌 和乳酸菌）的增殖均起到了显著的促进作用（P0.05），对于梭状菌和拟杆菌的增殖发挥了显著的抑制作用（P0.05），对肠道总菌群 数量影响不显著（P0.05）。此外对于发酵过程中各种短链脂肪酸含量的变化进行了测定，发现添加儿茶素样品的培养基中，短链脂 肪酸含量在各个发酵时间点都比对照组有显著提高（P0.05）。实验同时对于茶叶儿茶素发酵过程中的代谢产物进行了结构鉴定。研 究表明，包括甲基化儿茶素在内的儿茶素及其代谢产物，对于改善人体肠道环境、维护人体肠道平衡具有重要作用。 关键词：乌龙茶；茶叶儿茶素；短链脂肪酸；肠道微生态 文章篇号：1673-9078(2015)4-128-136 DOI: 10.13982/j.mfst.1673-9078.2015.4.021 In vitro Monitoring of the Probiotic Activity of Tea Catechins on Human Intestinal Flora YANG Yang, ZHANG Xin, WENG Pei-fang, WU Zu-fang (Department of Food Science, School of Marine Sciences, Ningbo University, Ningbo 315211, China) Abstract: Recently, tea catechins have been reported to have certain regulatory effect on intestinal microecology. In this study, high purity (98%) of EGCG, EGCG3’’Me, ECG and ECG3’’Me were prepared from Guangdong Oolong tea by puri?cation procedures of macroporous resins. Then, the regulation effects of EGCG, EGCG3’’Me, ECG and ECG3’’Me on intestinal microecology in vitro were evaluated by monitoring the bacterial populations and analyzing the concentrations of short-chain fatty acids (SCFA) at different fermentation times (0, 6, 12 and 24 h). The results showed that tea catechins had significant