Rapid differentiation of citrus Hop stunt viroid variants by real-time RT-PCR and high resolution melting analysis精品.pdf

Molecular and Cellular Probes 27 (2013) 221e229 Contents lists available at ScienceDirect Molecular and Cellular Probes journal homepage: www.els /locate/ymcpr Rapid differentiation of citrus Hop stunt viroid variants by real-time RT-PCR and high resolution melting analysis Giuliana Loconsole a, Nuket Önelge b, Raymond K. Yokomi c, Raied Abou Kubaa d, Vito Savino a, Maria Saponari e, * a Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari “Aldo Moro ”, Via Amendola 165/A, 70126 Bari, Italy b Çukurova University Agriculture Faculty, Plant Protection Department, 01330 Balcali Adana, Turkey c USDA, Agricultural Research Service, USDA-ARS San Joaquin Valley Agricultural Sciences Center, 9611 South Riverbend Avenue, Parlier, CA 93648-9757, USA d Department of Plant Protection, Ministry of Agriculture and Agrarian Reform, Damascus, Syria e Istituto di Virologia Vegetale, Consiglio Nazionale delle Ricerche, UOS Bari, Via Amendola 165/A, 70126 Bari, Italy a r t i c l e i n f o a b s t r a c t Article history: The RNA genome of pathogenic and non-pathogenic variants of citrus Hop stunt viroid (HSVd) differ by Received 23 May 2013 five to six nucleotides located within the variable (V) domain referred to as the “cachexia expression Received in revised form motif”. Sensitive hosts such as mandarin and its hybrids are seriously affected by cachexia disease. 19 July 2013 Current methods to differentiate HSVd variants rely on lengthy greenhouse biological indexing on Par- Accepted