原花青素对大鼠实验性血栓形成的影响.doc

原花青素对大鼠实验性血栓形成的影响 作者：名实医药研发中心 【摘要】本研究旨在探讨原花青素（PC）的抗血栓作用及其机制。麻醉动物，然后给颈动脉局部施以20%三氯化铁溶液 20分钟，以造成大鼠颈动脉血栓模型。实验分6组：假血栓（对照）组、血栓模型组、阿斯匹林［10 mg/（kg·d）］组、PC 100 mg/（kg·d）组、PC 200 mg/（kg·d）组及PC 400 mg/（kg·d）组。假血栓（对照）组和血栓模型组用生理盐水灌胃，其它各组分别用阿司匹林和PC低、中、高3个不同剂量悬液灌胃，每日2次，连续4周。观察各组药物对实验性血栓形成的大鼠血浆中的血栓烷（TXB2）和6酮前列腺素F1α（6 Keto PGF1α）及血小板P选择蛋白（GMP 140））含量的影响。结果发现： ①与假血栓对照组比较，血栓模型组血浆TXB2和血小板P选择蛋白（GMP 140）含量明显升高，血浆6 Keto PGF1α含量明显降低，差异非常显著（P＜0.01）；②与血栓模型组比较， 3个PC组和阿司匹林组大鼠血浆TXB2和血小板P选择蛋白（GMP 140）含量明显降低，差异非常显著（P＜0.01），血浆6 Keto PGF1α含量明显升高（P＜0.01）；③与阿司匹林组比较，3个PC组血浆TXB2和血小板P选择蛋白（GMP 140）含量均不同程度降低，而血浆6KetoPGF1α含量升高，其中尤以PC 400 mg/（kg·d）组最为显著（P＜0.01）。结论： PC具有明显的抗血栓形成作用，其抗血栓形成的机制与抑制血小板活化、聚集及保护血管内皮细胞密切相关。PC抗血栓形成作用有明显的剂量依赖性。PC 400 mg/（kg·d）组的抗血栓作用优于阿司匹林［10 mg/（kg·d）］组。 【关键词】 原花青素　血栓形成　血小板活化　血栓烷　6酮前列腺素F1α　血小板P选择蛋白 　　 Effects of Procyanidin Oligomers on Experimental Thrombosis in Rats Abstract The study was aimed to investigate the potential effect of procyanidins (PC) as а antithrombotic agent and its mechanism. 48 male SD rats were randomized into 6 groups, which include 8 rats each. Group A was normal control, group B was model control (no treatment), group C was group treated with aspirin ［10mg/（kg·d）］ , groups D, E and F were treated with low, medial and high dose ［100, 200 and 400 mg/（kg·d）］ of PC respectively. In accordance with Kurz′s protocols, rat′s model of thrombosis of common carotid artery was contructed with FeCl3, but for goup A 0.9% of normal saline was used for 20 minutes. The thromboxane B2 (TXB2), 6KetoPGF1α and GMP140 contents in plasma were measured. The results showed that compared with the normal control group, the contents of TXB2 and GMP140 in plasma markedly increased in all of PC groups and aspirin group, and the contents of 6KetoPGF1α in plasma decreased. Compared with the model group, the contents of TXB2 and GMP140 in plasma markedly decreased in all of PC groups and aspirin group, and the contents of 6KetoPGF1α in