乳杆菌对致病性大肠杆菌K88和O138体外存活抑制的动力学研究.PDF

微生物学通报 OCT 20, 2008, 35(10): 1606~1610 Microbiology © 2008 by Institute of Microbiology, CAS tongbao@ 研究报告 乳杆菌对致病性大肠杆菌K88 和O138 体外 存活抑制的动力学研究 林 勇 姚 文 朱伟云* ( 210095) 摘 要: 研究了一株分离自断奶仔猪小肠黏膜的肠乳杆菌L (Lactobacillus intestinalis)体外发酵特 1 性, 及其代谢产物对病原性大肠杆菌Escherichia coli K88 和O138 存活的影响。体外发酵结果表 明：发酵12 h 后, L1 菌液pH 值迅速降至 3.90, 并产生大量乳酸, 为 104.08 mmol/L。L1 菌株代谢 产物对K88 和O138 体外生长抑制的动力学研究表明：L1 菌株代谢产物对K88 和O138 存活具有 很强的抑制作用; L1 菌株发酵液与含相同浓度乳酸的自制培养液比较结果表明：乳酸在L1 菌株代 谢物对K88 和O138 存活抑制中发挥了主要作用; K88 和O138 对pH 4.5 的MRS 培养液具有一定 的耐受能力。 关键词: 肠乳杆菌, K88, O138, 生长抑制 Kinetic Analysis of the Inhibition of in vitro Growth of Pathogenic Escherichia coli K88 and O138 by Lactobacillus Isolates from Porcine Intestine * LIN Yong YAO Wen ZHU Wei-Yun (Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095) Abstract: Fermentation property and kinetic analysis of the inhibition of in vitro growth of pathogenic Es- cherichia coli K88, O138 by Lactobacillus intestinalis isolated from porcine intestine mucus were investi- gated. The results of fermentation showed that L1 result in a pH of 3.90 within 12 h and a large amount of lactic acid production, with the concentration of 104.08 mmol/L. Kinetic analysis of the antibacterial activity of L metabolic product toward K88, O138 showed that CFUS display strong antibacterial activity toward 1 K88, O138; the antibacterial activity of L1 CFUS was higher