cysteine dioxygenase 1 is a tumor suppressor gene silenced by promoter methylation in multiple human cancers半胱氨酸加双氧酶1是一个肿瘤抑制基因启动子甲基化沉默的多种人类癌症.pdf

Cysteine Dioxygenase 1 Is a Tumor Suppressor Gene Silenced by Promoter Methylation in Multiple Human Cancers 1,2 3 1 1 4 1 Mariana Brait , Shizhang Ling , Jatin K. Nagpal , Xiaofei Chang , Hannah Lui Park , Juna Lee , 1 5 1 1 Jun Okamura , Keishi Yamashita , David Sidransky *, Myoung Sook Kim * 1 Department of Otolaryngology, Head and Neck Cancer Research Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of ˆ America, 2 Clinical Research Coordination, Instituto Nacional de Cancer (INCA) - Brazilian National Cancer Institute, Rio de Janeiro, Rio de Janeiro, Brazil, 3 Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America, 4 Department of Epidemiology, University of California Irvine, Irvine, California, United States of America, 5 Department of Surgery, Kitasato University Hospital, Sagamihara, Kanagawa, Japan Abstract The human cysteine dioxygenase 1 (CDO1) gene is a non-heme structured, iron-containing metalloenzyme involved in the conversion of cysteine to cysteine sulfinate, and plays a key role in taurine biosynthesis. In our search for novel methylated gene promoters, we have analyzed differential RNA expression profiles of colorectal cancer (CRC) cell lines with or without treatment of 5-aza-29-deoxycytidine. Among the genes identified, the CDO1 promoter was found to be differentially methylated in primary CRC tissues with high frequency compared to normal colon tissues. In addition, a statistically signi