代谢组学与其应用.pdf

21 卷 1 期 生 　物 　工 　程 　学 　报 Vol . 21 　No . 1 2005 年 1 月 Chinese J ournal of B iotechnology J anuary 　2005 代谢组学及其应用 　 Metabonomics and its Applications 1 2 3 4 1 杨 　军 , 宋硕林 , Jose CastroPerez , Robert S. Plumb , 许国旺 YAN GJun1 , SON G ShuoLin2 , Jose CastroPerez3 , Robert S. Plumb4 and XU GuoWang1 1 中国科学院大连化学物理研究所 ,大连 　116011 1 N ational Chromatograp hic R . A . Center , Dalian Institute of Chemical Physics , Chinese Academy of Sciences , Dalian 　116011 , China 2 Nihon Waters K. K. ,140 0001 Toky o , J ap an 3 Waters Corporation , Manchester , UK 4 Waters Corporation , Mifl ord MA , USA 摘 　要 　对代谢组学的概念 、特性 、发展历史做了简要介绍 ,综述了当前代谢组学研究中的数据采集 、数据分析中采用的技 术 ,及代谢组学在疾病诊断、药物毒性研究、植物和微生物等领域的应用 ,并对代谢组学的发展作了展望 。 关键词 　代谢组学 , 靶标分析 , 代谢轮廓分析 , 指纹分析 , 综述 ( ) 中图分类号 　Q591 　　文献标识码 　A 　　文章编号 2005 Abstract 　The concept , characteristics and history of metabonomics are introduced . The techniques used in data acquisition and data analysis in metabonomics including their advantages and disadvantages are summarized . In data acquisition platform , NMR , GCMS , LCMS (MS) are the prevalent techniques although at present , none of them is a perfect technique that could meet with the requirement of the metabonomics for measuring all metabolites. While in data analysis , the PCA , PLS and ANN are the major techniques. The researchers could select them according to the research destination . Recent advances and applications of metabonomics in disease diagnosis , drug toxicity evaluation ,plant metabolomics