代谢组学方法与应用.pptx

MetabolomicsanditsapplicationsBaichuanDengUniversityofBergen邓百川2023最新整理收集do

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Contents1.Background2.Workflow3.Application4.Challenge

Contents1.Background2.Workflow3.Application4.Challenge

ManhattanProject(1942-1946)ApolloProject(1961-1972)HumanGenomeProject(1990-2003)HumanGenomeProject

The“omics”familyWeakWeakWeak

Humandiseaserisk

AdvantageofmetabolomicsLowComplexityNoninvasivebiofuidsHighSensitivityGenomicsProteomicsMetabolomicsMetabolicchangeEnzymaticchangeGenomicchangeAmplifyingUrineSerumPlasmaThetotalnumberofmetabolitesismuchlessthanthatofproteinsandgenesThestructureofmetabolitesismuchsimplerthanthatofproteinsandgenes

AliteraturesurveyinWebofScience(June10th,2015).‘Metabolomics’wasusedasakeyword.PublicationsandcitationsrelatedtometabolomicsYearYearNumberNumber(A)Publications(B)Citations

Contents1.Background2.Workflow3.Application4.Challenge

Alternateworkflows1Targetedmetabolomics2Nontargetedmetabolomics

Alternateworkflows1Targetedmetabolomics2NontargetedmetabolomicsSelectanalyticaltargetsSelectanalyticalmethodsConductexperimentsAnalyzesamplesandperformstatisticalanalysis

Alternateworkflows1Targetedmetabolomics2NontargetedmetabolomicsPosescientificquestionSamplecollectionHighresolutiondetectionanddataanalysisSignificantmetabolitesandpathways

WorkflowofmetabolomicsG.A.NaganaGowdaetal.ExpertRevMolDiagn.2008Keysteps:SamplecollectionSamplepreparationDataacquisitionDatapreprocessingStatisticalanalysisBiomarkeridentificationPathwayanalysis

WorkflowofmetabolomicsG.A.NaganaGowdaetal.ExpertRevMolDiagn.2008Keysteps:SamplecollectionSamplepreparationDataacquisitionDatapreprocessingStatisticalanalysisBiomarkeridentificationPathwayanalysisClinicTrials

WorkflowofNutritionalMetabolomics

Gaschromatography-massspectrometry(GC-MS)Nuclearmagneticresonance(NMR)Liquidchromatography-massspectrometry(LC-MS)Rete