人脐血间充质干细胞尾静脉移植治疗扩张型心肌病.docx

中国组织工程研究??2013,?Vol. 17??Issue (6): 1029-1036????DOI: 10.3969/j.issn.2095-4344.2013.06.014干细胞移植人脐血间充质干细胞尾静脉移植治疗扩张型心肌病Tail vein transplantation of human umbilical cord blood mesenchymal stem cells for treatment of dilated cardiomyopathy全文:?PDF?(681 KB)?摘要?背景：体外研究表明人脐血间充质干细胞可分化为自律性跳动的心肌细胞，而经静脉移植人脐血间充质干细胞治疗扩张型心肌病心力衰竭的报道较少。目的：探讨经尾静脉移植人脐血间充质干细胞对扩张型心肌病大鼠心肌结构、心功能的影响。方法：Wistar大鼠通过腹腔注射阿霉素诱导扩张型心肌病模型，扩张型心肌病实验组于造模后8周经尾静脉移植人脐血间充质干细胞，扩张型心肌病对照组注射等量DMEM培养基。健康对照组不造模，于相同时间点注射等体积的生理盐水。结果与结论：与健康对照组相比，扩张型心肌病组心功能明显受损，且扩张型心肌病实验组受损较扩张型心肌病对照组轻；移植的细胞有肌钙蛋白T的表达。结果提示人脐血间充质干细胞移植能促进扩张型心肌病大鼠心功能恢复，使心肌组织病变减轻。关键词?：?干细胞；?干细胞移植；?脐血间充质干细胞；?细胞培养；?细胞移植；?尾静脉\扩张型心肌病；?心功能；?心肌结构；?阿霉素；?干细胞图片文章； ??Abstract：BACKGROUND:?In vitro studies have demonstrated that human umbilical cord blood mesenchymal stem cells can differentiate into autorhythmic myocardial cells. Few studies are reported regarding tail vein transplantation of human umbilical cord blood mesenchymal stem cells for treatment of dilated cardiomyopathy.OBJECTIVE:?To investigate the influences of tail vein transplantation of human umbilical cord blood mesenchymal stem cells on myocardial structure and heart function in a rat model of dilated cardiomyopathy.METHODS:?Dilated cardiomyopathy was induced in Wistar rats by intraperitoneal injection of adriamycin. In the dilated cardiomyopathy group, human umbilical cord blood mesenchymal stem cells were injected into the rats via the tail vein at 8 weeks after dilated cardiomyopathy induction. In the model control group, equal amounts of DMEM were identically injected. In the blank control group, the rats were not subjected to dilated cardiomyopathy induction, but the same amount of physiological saline was injected at the same time point.RESULTS AND CONCLUSION:?Compared with the blank control group, the heart function of rats in the dilated cardiomyopathy group and model control group was significantly injured, and the disease severity was milder in the dilated cardiomyopathy group than in the model control g