complete sequencing of pndm-hk encoding ndm-1 carbapenemase from a multidrug-resistant escherichia coli strain isolated in hong kong完成测序的pndm-hk编码ndm - 1 .pdf

Complete Sequencing of pNDM-HK Encoding NDM-1 Carbapenemase from a Multidrug-Resistant Escherichia coli Strain Isolated in Hong Kong 1,2 1 1 3 1 1 Pak Leung Ho *, Wai U Lo , Man Kiu Yeung , Chi Ho Lin , Kin Hung Chow , Irene Ang , Amy Hin Yan 3 3 3 4 Tong , Jessie Yun-Juan Bao , Si Lok , Janice Yee Chi Lo 1 Department of Microbiology, The University of Hong Kong, Hong Kong, Special Administrative Region, People’s Republic of China, 2 Carol Yu Center for Infection, The University of Hong Kong, Hong Kong, Special Administrative Region, People’s Republic of China, 3 Genome Research Centre, The University of Hong Kong, Hong Kong, Special Administrative Region, People’s Republic of China, 4 Public Health Laboratory Services Branch and Department of Health, Centre for Health Protection, Hong Kong, Special Administrative Region, People’s Republic of China Abstract Background: The emergence of plasmid-mediated carbapenemases, such as NDM-1 in Enterobacteriaceae is a major public health issue. Since they mediate resistance to virtually all b-lactam antibiotics and there is often co-resistance to other antibiotic classes, the therapeutic options for infections caused by these organisms are very limited. Methodology: We characterized the first NDM-1 producing E. coli isolate recovered in Hong Kong. The plasmid encoding the metallo-b-lactamase gene was sequenced. Principal Findings: The plasmid, pNDM-HK readily transferred to E. coli J53 at high frequencies. It belongs to the broad host range IncL/M incompatibility group and is 88803 bp in size. Sequence alignment showed that pNDM-HK has a 55 kb backbone which shared 97% homology with pEL60 originating fr