the carotenogenesis pathway via the isoprenoid-β-carotene interference approach in a new strain of dunaliella salina isolated from baja california mexico主要通路通过isoprenoid-β-carotene干扰方法的新菌株杜氏隔绝墨西哥下加利福尼亚.pdf

Mar. Drugs 2009, 7, 45-56 doi:10.3390/md7010045 OPEN ACCESS Marine Drugs ISSN 1660-3397 /journal/marinedrugs Article The Carotenogenesis Pathway via the Isoprenoid-β-carotene Interference Approach in a New Strain of Dunaliella salina Isolated from Baja California Mexico J. Paniagua-Michel 1,*, Willian Capa-Robles 2 , Jorge Olmos-Soto 1, Luis Enrique Gutierrez- 1 Millan 1 Department of Marine Biotechnology, Centro de Investigación Científica y de Educación Superior de Ensenada, Ensenada 22860, México 2 Present address: Facultad de Ciencias, Universidad Nacional del Santa. Av. Universitaria S/N, Bellamar, Chimbote, Peru * Author to whom correspondence should be addressed; Tel. +52-646-1750500; Fax: +52-646- 1750569; E-mail: jpaniagu@cicese.mx. Received: 5 August 2008; in revised: 5 January 2009 / Accepted: 19 January 2009 / Published: 10 February 2009 Abstract: D. salina is one of the recognized natural sources to produce β-carotene, and an useful model for studying the role of inhibitors and enhancers of carotenogenesis. However there is little information in D. salina regarding whether the isoprenoid substrate can be influenced by stress factors (carotenogenic) or selective inhibitors which in turn may further contribute to elucidate the early steps of carotenogenesis and biosynthesis of β-carotene. In this study, Dunaliella salina (BC02) isolated from La Salina BC Mexico, was subjected to the method of isoprenoids-β-carotene interference in order to