colloids as mobile substrates for the implantation and integration of differentiated neurons into the mammalian brain胶体作为移动基质分化神经元的移植和集成到哺乳动物大脑.pdf

Colloids as Mobile Substrates for the Implantation and Integration of Differentiated Neurons into the Mammalian Brain 1 3 3 3¤ 3 Dennis Jgamadze , Jamie Bergen , Daniel Stone , Jae-Hyung Jang , David V. Schaffer , Ehud Y. 2 1 Isacoff *, Sophie Pautot * 1 Center for Regenerative Therapies Dresden, Dresden, Germany, 2 Department of Molecular and Cell Biology, University of California, Berkeley, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America, 3 Department of Chemical Engineering, University of California, Berkeley, California, United States of America Abstract Neuronal degeneration and the deterioration of neuronal communication lie at the origin of many neuronal disorders, and there have been major efforts to develop cell replacement therapies for treating such diseases. One challenge, however, is that differentiated cells are challenging to transplant due to their sensitivity both to being uprooted from their cell culture growth support and to shear forces inherent in the implantation process. Here, we describe an approach to address these problems. We demonstrate that rat hippocampal neurons can be grown on colloidal particles or beads, matured and even transfected in vitro, and subsequently transplanted while adhered to the beads into the young adult rat hippocampus. The transplanted cells have a 76% cell survival rate one week post-surgery. At this time, most transplanted neurons have left their beads and elaborated long processes, similar to the host neurons. Additionally, the transplanted cells distribute uniformly across the host hippocampus. Expression of a fluorescent protein and