splunc1及其bpi结构域缺失突变体对鼻咽癌细胞基因表达谱以及信号通路影响初步研究.pdf

墨苎塑至 ～一 Proceededfrom BPIdomain ofSPLUNC1 inthis studv’aBPI deletedSPLUNC1 mutant was TA designed and by cloningPCR theobservationof amplification．Through GFP．SPLUNC I．△BPI fusion inHNEl protein expression cells，wediscoveredthattheBPI domainisofthe effects onthe important sub．10calizationof SPLUNC1 inHNElceils．The protein GFP—SPLUNCl。ABPI fusionDroteinis distributedin evenly and cytoplasm GFP’SPLUNC1 fusion was to protein some aggregated intracellular ZhouinOllr puncdform labhadfound aggregates．Dr．Houde thatthese punctiform are aggregates thecell nanobacteria(5n3)．Becausewallof NBis of BPIdomain LPS，the isthe composed theoreticalLPS binding that BPIdomain domain，wehypothesis Can the guideSPLUNC1protein to