玉米酒精发酵前提取超氧化物歧化酶的研究.pdf

2 1 6 V ol . 2 1N o . 6 17 6 2005 6 T r ansaction s of the CSA E J une2005 赵　华, 陶　静 ( , 300222) , ( SO D) , 2 0. 05 / ( 7. 8) , 40 36 SO D m ol L pH h SO D 0. 05 m ol / L ( pH 7. 8) , 21( v/ m) , , 1 h 40% , 90% , SO D , 168. 3 U/ mg 超氧化物歧化酶; 提取; 玉米; 酒精发酵 Q 554. 6; S 5 13 B 1002 -6 819( 2005) 06-0 17 6-04 , . [ J ] . , 2005, 21( 6) 176- 179. Zh ao Hu a, T a o J ing . Ext raction o f super o xide dismutase fro m m aize bef or e et ha no l f erm enta tio n pr o cessing [ J ] . T r an sa ct ion s of t he CSA E , 2005, 21( 6) 17 6- 179. ( in Chinese w ith English ab st ract) 100 g , p H 7 . 8 0 . 05 mol/ L 0 , ; ( N H ) SO 40% , , , ( sup ero x ide dismutase, SOD ; E C 1. 15 . 1. 1) 4 2 4 ( NH ) SO 90% , , 4 2 4 , SO D ( . - ) , . - [ 1] , 1. 3 . 2　SO D 酶活力的测定 O 2 O 2 H2 O2 O 2 , SO D SO D [ 12, 13] [ 2] 1 m L 50 % , [ 3, 4] ( U ) ; [ 5] ( U / mg ) , SO D , , 1. 3 . 3　蛋白质含量的测定 [ 14] [ 6] F olin - ( ) ( ) ( ) [ 7- 9] 1. 3 . 4