spectral counting assessment of protein dynamic range in cerebrospinal fluid following depletion with plasma-designed immunoaffinity columns光谱计数对脑脊液蛋白质动态范围的评估后损耗与plasma-designed immunoaffinity列.pdf

CLINICAL PROTEOMICS Spectral counting assessment of protein dynamic range in cerebrospinal fluid following depletion with plasma-designed immunoaffinity columns Borg et al. Borg et al. Clinical Proteomics 2011, 8:6 /content/8/1/6 (3 June 2011) Borg et al. Clinical Proteomics 2011, 8:6 /content/8/1/6 CLINICAL PROTEOMICS RESEARCH Open Access Spectral counting assessment of protein dynamic range in cerebrospinal fluid following depletion with plasma-designed immunoaffinity columns 1* 2 3 3 2 4 Jacques Borg , Alex Campos , Claudio Diema , Núria Omeñaca , Eliandre de Oliveira , Joan Guinovart and Marta Vilaseca3 * Correspondence: Jacques. Abstract Borg@univ-st-etienne.fr 1Laboratoire de Neurobiochimie, Background: In cerebrospinal fluid (CSF), which is a rich source of biomarkers for Université Jean Monnet, Saint- Etienne, France neurological diseases, identification of biomarkers requires methods that allow Full list of author information is reproducible detection of low abundance proteins. It is therefore crucial to decrease available at the end of the article dynamic range and improve assessment of protein abundance. Results: We applied LC-MS/MS to compare the performance of two CSF enrichment techniques that immunodeplete either albumin alone