spectral counting assessment of protein dynamic range in cerebrospinal fluid following depletion with plasma-designed immunoaffinity columns光谱计数对脑脊液蛋白质动态范围的评估后损耗与plasma-designed immunoaffinity列.pdf
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CLINICAL
PROTEOMICS
Spectral counting assessment of protein dynamic
range in cerebrospinal fluid following depletion
with plasma-designed immunoaffinity columns
Borg et al.
Borg et al. Clinical Proteomics 2011, 8:6
/content/8/1/6 (3 June 2011)
Borg et al. Clinical Proteomics 2011, 8:6
/content/8/1/6 CLINICAL
PROTEOMICS
RESEARCH Open Access
Spectral counting assessment of protein dynamic
range in cerebrospinal fluid following depletion
with plasma-designed immunoaffinity columns
1* 2 3 3 2 4
Jacques Borg , Alex Campos , Claudio Diema , Núria Omeñaca , Eliandre de Oliveira , Joan Guinovart and
Marta Vilaseca3
* Correspondence: Jacques. Abstract
Borg@univ-st-etienne.fr
1Laboratoire de Neurobiochimie,
Background: In cerebrospinal fluid (CSF), which is a rich source of biomarkers for
Université Jean Monnet, Saint-
Etienne, France neurological diseases, identification of biomarkers requires methods that allow
Full list of author information is reproducible detection of low abundance proteins. It is therefore crucial to decrease
available at the end of the article dynamic range and improve assessment of protein abundance.
Results: We applied LC-MS/MS to compare the performance of two CSF enrichment
techniques that immunodeplete either albumin alone
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