农林牧渔PlantGenomicsMethodsandProtocols.doc

Plant Genomics Methods and Protocols Edited by Daryl J. Somers*, Peter Langridge?, and J. Perry Gustafson? Molecular Breeding and Biotechnology, Vineland Research and Innovation Centre, Vineland Station, Ontario, Canada* Australian Centre for Plant Functional Genomics, University of Adelaide, Glen Osmond, Australia? Division of Plant Sciences, University of Missouri, Columbia, MO, USA? Preface This volume is divided into chapters which consider the primary issues and methodologies surrounding plant genomics research. Plant genomics is largely concerned with associating functional genes or gene mutations with phenotype. Therefore, chapters areincluded that cover the areas of gene discovery and functional analysis of genes. Furthe rchapters focus on the primary tools and sub-disciplines of genetic mapping, mRNA,protein and metabolite profiling. Methods are included that explore gene functional analysis via transformation, mutation, protein function and gene expression. The volume includes chapters on data management which consider the expansion of plant genomics databases and bioinformatics analysis tools. The volume is concluded with chapters aimed at discussing the application and deployment of molecular plant breeding technology from the use of markers in breeding, development of genetically modified plants/crop species, analysis of existing populations for novel alleles and gene/trait associations and genome sequencing. Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix 1 Role of Model Plant Species . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Richard Flavell 2 New Technologies for Ultra-High Throughput Genotyping in Plants . . . . . . . . . . 19 Nikki Appleby, David Edwards, and Jacqueline Batley 3 Genetic Maps and