H.A刺激对抗坏血酸过氧化物酶基因表达的影响.pdf

Plant Cell Rep (2010) 29:1169–1178 DOI 10.1007/s00299-010-0903-z ORIGINAL PAPER Analysis of ascorbate peroxidase genes expressed in resistant and susceptible wheat lines infected by the cereal cyst nematode, Heterodera avenae • • • ´ ´ Ester Simonetti Eva Alba Marıa Jesus Montes ´ • ´ ˜ Angeles Delibes Isidoro Lopez-Brana Received: 22 March 2010 / Revised: 28 June 2010 / Accepted: 18 July 2010 / Published online: 6 August 2010 Springer-Verlag 2010 Abstract Changes in ascorbate peroxidase (APX) enzyme Abbreviations activity in response to nematode (Heterodera avenae) attack APX Ascorbate peroxidase were studied in roots of three hexaploid wheat lines carrying AsA Ascorbic acid Cre2, Cre5, or Cre7 nematode resistance genes and the Cre Cereal root eelworm susceptible Triticum aestivum cv. Anza. A spectrophoto- CCN Cereal cyst nematode metric analysis was carried out with root extracts of infected dai Days after inoculation plants 4, 7, 11, and 14 days after nematode inoculation using EST Esterase uninfected plant as control. APX induction in infected GSH Reduced form of glutathione resistant genotypes was similar and higher than in the sus- PRX Plant class III peroxidases ceptible control. The introgression wheat/Aegilops ventri- ROS Reactive oxygen species cosa H-93-8 line, carrying the Cre2 gene, and its parental SOD Superoxide dismutase line H-10-15 as susceptible control were used to analyze whether this increase of activity was correlated with the induction of