xenopus as a model system for the study of golph2gp73 function xenopus golph2 is required for pronephros development非洲爪蟾蜍作为一个模型系统研究golph2gp73函数非洲爪蟾蜍golph2前肾需要发展.pdf
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Xenopus as a Model System for the Study of GOLPH2/
GP73 Function: Xenopus golph2 Is Required for
Pronephros Development
1,2 3,4 1 1,2 1 3 1,2
Leike Li , Luan Wen , Yu Gong , Guoqiang Mei , Jinsong Liu , Yonglong Chen *, Tao Peng *
1 State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China, 2 University of Science
and Technology of China, Hefei, China, 3 Key Laboratory of Regenerative Biology, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences,
Guangzhou, China, 4 Section on Molecular Morphogenesis, Laboratory of Gene Regulation and Development, Program in Cellular Regulation and Metabolism, Eunice
Kennedy Shriver National Institute Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America
Abstract
GOLPH2 is a highly conserved protein. It is upregulated in a number of tumors and is being considered as an emerging
biomarker for related diseases. However, the function of GOLPH2 remains unknown. The Xenopus model is used to study the
function of human proteins. We describe the isolation and characterization of Xenopus golph2, which dimerizes and
localizes to the Golgi in a manner similar to human GOLPH2. Xenopus golph2 is expressed in the pronephros during early
development. The morpholino-mediated knockdown of golph2 results in edema formation. Additionally, Nephrin
expression is enhanced in the glomus, and the expression of pronephric marker genes, such as atp1b1, ClC-K, NKCC2, and
NBC1, is diminished in the tubules and duct. Expression patterns of the transcript
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