continued neurogenesis in adult drosophila as a mechanism for recruiting environmental cue-dependent variants持续的神经发生在成人果蝇作为招聘的机制环境cue-dependent变体.pdf

Continued Neurogenesis in Adult Drosophila as a Mechanism for Recruiting Environmental Cue- Dependent Variants Selim Ben Rokia-Mille, Sylvette Tinette, Gilbert Engler, Laury Arthaud, Sophie Tares, Alain Robichon* Department of Biology and Biotic Interactions, CNRS/INRA, University Nice Sophia-Antipolis, Sophia Antipolis, France Abstract Background: The skills used by winged insects to explore their environment are strongly dependent upon the integration of neurosensory information comprising visual, acoustic and olfactory signals. The neuronal architecture of the wing contains a vast array of different sensors which might convey information to the brain in order to guide the trajectories during flight. In Drosophila, the wing sensory cells are either chemoreceptors or mechanoreceptors and some of these sensors have as yet unknown functions. The axons of these two functionally distinct types of neurons are entangled, generating a single nerve. This simple and accessible coincidental signaling circuitry in Drosophila constitutes an excellent model system to investigate the developmental variability in relation to natural behavioral polymorphisms. Methodology/Principal Findings: A fluorescent marker was generated in neurons at all stages of the Drosophila life cycle using a highly efficient and controlled genetic recombination system that can be induced in dividing precursor cells (MARCM system, flybase web site). It allows fluorescent signals in axons only when the neuroblasts and/or neuronal cell precursors like SOP (sensory organ precursors) undergo division during the precedent steps. We first show that a robust neurogenesis continues in the wing after the adults emerge from the pupae followed by an extensive axonal growth. Arguments are presented to suggest that this wing neurogenesis