spectroscopicmethodstostudytheinteractionofbovineserumalbuminandAg光谱学研究牛血清白蛋白.pdf

Science of the Total Environment 409 (2011) 892–897 Contents lists available at ScienceDirect Science of the Total Environment journal homepage: /locate/scitotenv The interaction between Ag + and bovine serum albumin: A spectroscopic investigation Xingchen Zhao, Rutao Liu ⁎, Yue Teng, Xiaofang Liu Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, China–America CRC for Environment Health, Shandong Province, 27# Shanda South Road, Jinan 250100, PR China a r t i c l e i n f o a b s t r a c t Article history: By using spectroscopic methods, we probed the interaction of Ag+ with bovine serum albumin (BSA) in an Received 16 April 2010 aqueous environment. Fluorescence of BSA quenched by Ag+ is a dynamic quenching process. Two binding Received in revised form 31 October 2010 modes–a strong one at low concentration of Ag+ and a weak one at high concentration were found. The Accepted 5 November 2010 3 − 1 association constant (KA ) and the number of binding sites (n) were 4.88 ×10 M and 1.17 for strong Available online 17 December 2010 − 1 θ binding, and 17.6 M and 0.547 for weak binding at 293 K. The results