construction of vascular tissues with macro-porous nano-fibrous scaffolds and smooth muscle cells enriched from differentiated embryonic stem cells血管组织建设与macro-porous nano-fibrous支架和平滑肌细胞丰富从胚胎干细胞分化.pdf

Construction of Vascular Tissues with Macro-Porous Nano-Fibrous Scaffolds and Smooth Muscle Cells Enriched from Differentiated Embryonic Stem Cells 1. 2. 1 3 1,4,5,6 2 Jiang Hu , Changqing Xie , Haiyun Ma , Bo Yang , Peter X. Ma *, Y. Eugene Chen * 1 Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, Michigan, United States of America, 2 Department of Internal Medicine, Cardiovascular Center, University of Michigan, Ann Arbor, Michigan, United States of America, 3 Department of Cardiac Surgery, Cardiovascular Center, University of Michigan, Ann Arbor, Michigan, United States of America, 4 Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, United States of America, 5 Macromolecular Science and Engineering Center, University of Michigan, Ann Arbor, Michigan, United States of America, 6 Department of Materials Science and Engineering, University of Michigan, Ann Arbor, Michigan, United States of America Abstract Vascular smooth muscle cells (SMCs) have been broadly used for constructing tissue-engineered blood vessels. However, the availability of mature SMCs from donors or patients is very limited. Derivation of SMCs by differentiating embryonic stem cells (ESCs) has been reported, but not widely utilized in vascular tissue engineering due to low induction efficiency and, hence, low SMC purity. To address these problems, SMCs were enriched from retinoic acid induced mouse ESCs with LacZ genetic labeling under the control of SM22a promoter as the positive sorting marker in the present study. The sorted SMCs were characterized and then cultured on three-dimensional macro-porous nano-fibrous scaffolds in vitro or implanted subcutaneo