乙酰肝素酶的表达与胃癌浸润与淋巴结转移的关系.pdf

Modern Pathology (2005) 18, 205–211 2005 USCAP, Inc All rights reserved 0893-3952/05 $30.00 Positive association of heparanase expression with tumor invasion and lymphatic metastasis in gastric carcinoma 1,2 2 1 2 2 1,3 Zhenning Wang , Huimian Xu , Li Jiang , Xin Zhou , Chong Lu and Xue Zhang 1The Research Center for Medical Genomics and MOH Key Laboratory of Cell Biology; 2Department of Surgical Oncology, the First Hospital of China Medical University, Shenyang, PR China and 3Department of Medical Genetics and National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, PR China Tumor invasion and metastasis are the most common causes of death in gastric carcinoma. Human heparanase influences tumor invasiveness and angiogenesis. Analysis of its expression in gastric carcinoma has been hindered by our inability to procure pure cancer cells from heterogeneous tissue. In the present study, we analyzed heparanase expression in human primary and metastatic gastric carcinoma cells as well as in paired normal gastric epithelial cells by laser capture microdissection coupled with reverse transcription- polymerase chain reaction (RT-PCR). Tumor tissues, metastatic lymph nodes, and apparently uninvolved normal gastric tissues were collected from 30 patients who had undergone gastrectomy with radical lymph node dissection for gastric carcinoma without preoperative treatment. Bulk tissues and laser cap