targeted p120-catenin ablation disrupts dental enamel development针对p120-catenin消融破坏牙釉质的发展.pdf

Targeted p120-Catenin Ablation Disrupts Dental Enamel Development 1,2 1 1,2 3 4 John D. Bartlett *, Justine M. Dobeck , Coralee E. Tye , Mirna Perez-Moreno , Nicole Stokes , Albert B. 5 4 1 Reynolds , Elaine Fuchs , Ziedonis Skobe 1 Department of Cytokine Biology, Forsyth Institute, Boston, Massachusetts, United States of America, 2 Department of Developmental Biology, Harvard School of Dental Medicine, Boston, Massachusetts, United States of America, 3 Centro Nacional de Investigaciones Oncologicas, Madrid, Spain, 4 Howard Hughes Medical Institute, Rockefeller University, New York, New York, United States of America, 5 Department of Cancer Biology, School of Medicine, Vanderbilt University, Nashville, Tennessee, United States of America Abstract Dental enamel development occurs in stages. The ameloblast cell layer is adjacent to, and is responsible for, enamel formation. When rodent pre-ameloblasts become tall columnar secretory-stage ameloblasts, they secrete enamel matrix proteins, and the ameloblasts start moving in rows that slide by one another. This movement is necessary to form the characteristic decussating enamel prism pattern. Thus, a dynamic system of intercellular interactions is required for proper enamel development. Cadherins are components of the adherens junction (AJ), and they span the cell membrane to mediate attachment to adjacent cells. p120 stabilizes cadherins by preventing their internalization and degradation. So, we asked if p120-mediated cadherin stability is important for dental enamel formation. Targeted p120 ablation in the mouse enamel organ had a striking eff