Demo Data Analysis Procedure：演示数据分析程序.doc

Demo: Data Analysis ProcedureThis demo uses a simple and artificial microarray dataset of 10 breast cancer patients to demonstrate some data analysis steps utilized in this study. Table 1 and 2 separately list the clinical data of all patients and the expression measurements of all sequences in the source dataset. The original study annotated the sequences using accessions of NCBI RefSeq database and has processes microarray images to generate a 2-dimensional matrix of gene expression data. The expression profiling procedure used in this study would start with the categorization of sample patients into prognosis groups.Table SEQ Table \* ARABIC 1 Clinical Information of Sample PatientsSample IDRecurrenceFollow-up(yr)ER StatusTumor SizeGradeAge(yr)p_112.5312243p_206.4412144p_311.6602341p_411.3012341p_5011.9802348p_611.1611249p_7010.1402146p_808.8002348p_901.2911348p_1016.6411238 Table SEQ Table \* ARABIC 2 Expression Measurements in Source DatasetSequence IDp_1p_2p_3p_4p_5p_6p_7p_8p_9p_10NM_0030000.03-0.090.080.08-0.010.090.100.00-0.02-0.08NM_0030011.11-0.121.280.201.11-0.041.07-0.131.02-0.14NM_003002-0.26-0.17-0.35-0.26-0.290.03-0.060.12-0.27-0.13NM_003003-0.70-0.04-0.73-0.05-0.71-0.03-0.63-0.01-0.690.03NM_0030040.820.250.60-0.080.780.160.63-0.230.650.07NM_003005-0.890.01-0.810.05-0.800.01-0.60-0.02-0.770.13NM_003006-0.78-0.01-0.710.13-0.86-0.09-0.70-0.01-0.76-0.03NM_003007-1.230.25-1.090.23-1.230.05-1.100.04-1.160.45NM_003033-1.290.25-1.110.26-1.34-0.01-1.160.08-1.190.44NM_1981391.09-0.151.06-0.181.210.001.16-0.070.95-0.34A.1 Categorization of Sample PatientsBreast cancer patients are categorized into prognosis groups based on their follow-up data. Patients who had observed recurrence within three years after diagnosis are classified into poor prognosis group (p_1, p_3, p_4, and p_6). Patients who were followed up for at least three year and had no observed recurrence are classified into good prognosis group (p_2, p_5, p_7, and p_8). The follow-up of p_9 was too