arabidopsis nmd3 is required for nuclear export of 60s ribosomal subunits and affects secondary cell wall thickening拟南芥nmd3的核出口需要60年代核糖体亚基,影响次生细胞壁增厚.pdf

Arabidopsis NMD3 Is Required for Nuclear Export of 60S Ribosomal Subunits and Affects Secondary Cell Wall Thickening 1 2 3 4 3 1 1 3 Mei-Qin Chen , Ai-Hong Zhang , Quan Zhang , Bao-Cai Zhang , Jie Nan , Xia Li , Na Liu , Hong Qu , 2 3 4 1 1 Cong-Ming Lu , Sudmorgen , Yi-Hua Zhou , Zhi-Hong Xu , Shu-Nong Bai * 1 PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University and The National Center of Plant Gene Research, Beijing, China, 2 Institute of Botany, Chinese Academy of Sciences, Beijing, China, 3 College of Life Sciences, Peking University, Beijing, China, 4 State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China Abstract NMD3 is required for nuclear export of the 60S ribosomal subunit in yeast and vertebrate cells, but no corresponding function of NMD3 has been reported in plants. Here we report that Arabidopsis thaliana NMD3 (AtNMD3) showed a similar function in the nuclear export of the 60S ribosomal subunit. Interference with AtNMD3 function by overexpressing a truncated dominant negative form of the protein lacking the nuclear export signal sequence caused retainment of the 60S ribosomal subunits in the nuclei. More interestingly, the transgenic Arabidopsis with dominant negative interference of AtNMD3 function showed a striking failure of secondary cell wall thickening, consistent with the altered expression of related genes and composition of cell wall components. Obser