the essentials of protein import in the degenerate mitochondrion of entamoeba histolytica蛋白质的基本进口痢疾阿米巴的退化线粒体.pdf

The Essentials of Protein Import in the Degenerate Mitochondrion of Entamoeba histolytica 1,2,3¤* 1 4 2 ´2 1 Pavel Dolezal , Michael J. Dagley , Maya Kono , Peter Wolynec , Vladimir A. Likic , Jung Hock Foo , ´ 3 3 4 4 1 Miroslava Sedinova , Jan Tachezy , Anna Bachmann , Iris Bruchhaus , Trevor Lithgow 1 Department of Biochemistry and Molecular Biology, Monash University, Clayton Campus, Melbourne, Victoria, Australia, 2 Bio21 Institute, University of Melbourne, Parkville, Victoria, Australia, 3 Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic, 4 Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany Abstract Several essential biochemical processes are situated in mitochondria. The metabolic transformation of mitochondria in distinct lineages of eukaryotes created proteomes ranging from thousands of proteins to what appear to be a much simpler scenario. In the case of Entamoeba histolytica, tiny mitochondria known as mitosomes have undergone extreme reduction. Only recently a single complete metabolic pathway of sulfate activation has been identified in these organelles. The E. histolytica mitosomes do not produce ATP needed for the sulfate activation pathway and for three molecular chaperones, Cpn60, Cpn10 and mtHsp70. The already characterized ADP/ATP carrier would thus be essential to provide cytosolic ATP for these processes, but how the equilibrium of inorganic phosphate could be maintained was unknown. Finally, how the mitosomal proteins are translocated to the mitosomes had remained