乳酸杆菌表层蛋白及应用研究.pdf

※专题论述 食品科学 2010, Vol. 31, No. 03 2 79 乳酸杆菌表层蛋白及应用研究 刘元元，杨秀华，王远亮，李宗军* (湖南农业大学食品科技学院，食品科学与生物技术湖南省重点实验室，湖南长沙 4 10 12 8) 摘 要：在一些古细菌和真细菌中，表层蛋白是细胞膜最外层的结构。尽管所有表层蛋白具有相同的氨基酸成分， 但是与其他细菌表层蛋白相比，乳酸杆菌表层蛋白具有其独有的特性：小分子量，高等电点，为碱性蛋白。一 些乳酸杆菌具有多个表层蛋白基因。国外学者对表层蛋白作为功能分子共价吸附固定的基质、表面展示载体和分子 筛等方面的应用进行了深入研究，展示了巨大的发展前景。 关键词：表层蛋白；乳酸杆菌；黏附；生物分析；表面展示 Surface Layer Protein from Lactobacillus spp. and Its Application: A Review LIU Yuan-yuan ，YANG Xiu-hua ，WANG Yuan-liang ，LI Zong-jun * (Food Science and Biotechnology Key Laboratory of Hunan Province, College of Food Science and Technology, Hunan Agricultural University, Changsha 4 10 128, China) Abstract ：S-layer (surface layer) has been identified as the outermost structure of cellular envelopes in numerous organisms from bacteria and archaea domains. Despite high similarity of amino acid composition in all known S-layer proteins, the typical characteristics of S-layer proteins from Lactobacillus are small molecular weight, high isoelectric point and alkaline protein, which are distinguished from other S-layer proteins. Several species of Lactobacilli contain multiple S-layer protein genes. Inten sive attentions have been paid to the applications of surface layer proteins from Lactobacillus, including applications as a functional molecule to covalently absorb fixed matrix, as a carrier of cellular surface display and as a molecular sieve. Therefore, it should have tremendous development perspectives. Key words ：surface layer protein ；L actobacillus ；adhesion ；bioassay ；surface display 中图分类号：Q8 16 文献标识码：A 文章编号：1002-6630(20 10)03-0279-05 细胞壁是原核生物的重要组成成分，绝大多数真细 正方形或对称的六边形等多种形状，它是在内部的自我 菌和古细菌都具有较清晰的细胞壁