wolbachia-mediated cytoplasmic incompatibility is associated with impaired histone deposition in the male pronucleuswolbachia-mediated细胞质不相容与组蛋白沉积在雄性原核受损.pdf

Wolbachia-Mediated Cytoplasmic Incompatibility Is Associated with Impaired Histone Deposition in the Male Pronucleus ´ ´ 1 2 2 1 Frederic Landmann , Guillermo A. Orsi , Benjamin Loppin , William Sullivan * ´ ´ 1 Department of Molecular, Cell, and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America, 2 Centre de Genetique ´ ´ Moleculaire et Cellulaire, CNRS UMR5534, Universite Lyon 1, Lyon, France Abstract Wolbachia is a bacteria endosymbiont that rapidly infects insect populations through a mechanism known as cytoplasmic incompatibility (CI). In CI, crosses between Wolbachia-infected males and uninfected females produce severe cell cycle defects in the male pronucleus resulting in early embryonic lethality. In contrast, viable progeny are produced when both parents are infected (the Rescue cross). An important consequence of CI–Rescue is that infected females have a selective advantage over uninfected females facilitating the rapid spread of Wolbachia through insect populations. CI disrupts a number of prophase and metaphase events in the male pronucleus, including Cdk1 activation, chromosome condensation, and segregation. Here, we demonstrate that CI disrupts earlier interphase cell cycle events. Specifically, CI delays the H3.3 and H4 deposition that occurs immediately after protamine removal from the male pronucleus. In addition, we find prolonged retention of the replication factor PCNA in the male pronucleus into metaphase, indicating progression into mitos