利用鸡F2资源群体构建1号染色体遗传连锁图谱.pdf

HEREDITAS (Beijing) 2007 8 , 29(8): 977―981 ISSN 0253-9772 研究报告 DOI: 10.1360/yc-007-0977 F2 1 1 1 2 2 1 1 2 1 柳晓峰 , 王守志 , 胡晓湘 , 高宇 , 王启贵 , 张慧 , 李宁 , 李辉 1. , 150030; 2. , 100094 : 在鸡 1 号染色体上选取23 个微卫星标记，利用东北农业大学鸡F2 资源群体构建了遗传连锁图谱。选用 369 只F2 个体用于基因型测定。结果表明23 个微卫星位点除MCW0058 为低度多态，其他位点均为中高度多 态。构建的连锁图谱覆盖 1 号染色体全长，总共637.9 cM 。MCW0115 和ROS0025 标记顺序与EL 图谱不同， 但与WAU 图谱一致。其他标记顺序与3 大参考家系标记顺序一致，图谱总长和标记间距离大于3 大参考家系。 此连锁图谱的构建为数量性状位点（QTL ）定位奠定了良好的基础。 : 鸡; F2 资源群体; 微卫星; 连锁图谱 A genetic linkage map of chicken chromosome 1 in an F2 resource population 1 1 2 2 1 LIU Xiao-Feng , WANG Shou-Zhi , HU Xiao-Xiang , GAO Yu , WANG Qi-Qui , 1 2 1 ZHANG Hui , LI Ning , LI Hui 1. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China; 2. National Laboratories for Agribiotechnology , China Agricultural University, Beijing 100094, China Abstract: Three hundred and sixty-nine F individuals produced from Northeast Agricultural University Resource Popula- 2 tion (NEAURP) were genotyped by 23 fluorescent microsatellite markers on chromosome 1. The characterization of these microsatellites was moderate or high polymorphic except marker MCW0058 which was low polymorphic. The length of the sex averaged linkage map was 637.9 cM. The order of MCW0115 and ROS0025 disagreed with that of EL map, but con- sisted with that of W map. The intervals of markers were larger than those of three reference families. Keywords: chicken; F resource population; microsatellites; genetic linkage map