NEPA21活体转染的应用及参数设置.pdf

[Mouse/Rat] In Utero Gene transfer into embryonic brains using in utero electroporation technique Cultured Electroporation for mammalian embryos in the whole embryo culture system Embryo Brain Slice Electroporation-mediated gene transfer system applied to cultured CNS neurons Adult Brain Electroporation-mediated gene transfer in the adult rat brain Newborn Retina Protocol for in vivo electroporation into mouse/rat retina Muscle Gene transfer into muscle by electroporation in vivo Skin Epidermis-Targeted Gene Transfer Using In Vivo Electroporation Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice Testis with a Sertoli Cell Defect [Chick] In Ovo Misexpression of the gene of interest by in ovo electroporation Knock-down by transfection of shRNA expression vector by in ovo In Ovo electroporation EmbryoTissue Electroporation for early chick embryos using News culture (gastrula) Method to introduce genes into epithelial cells of the chicken embryonic stomach Embryo Tissue (proventriculus) Plant Seed Direct Gene Transfer into Mature Seeds via Electroporation Electroporation of Xenopus embryo: Gene Delivery into the Eye Primordium at Xenopus the Neural Plate Stage Honeybee In vivo gene transfer into the adult honeybee brain by using electroporation Cancer Therapy Electrochemotherapy for digital chondrosarcoma Single Cell Gene transfer into single cell by electroporation [In Utero] Gene transfer into embryonic brains using in utero electroporation technique Instruments: Fig. A In Vivo Electroporator: NEPA21, CUY21SC or CUY21EDIT (Nepa Gene Co., Ltd.) In Vivo Electrode (Nepa Gene Co., Ltd.) CUY650P3 (Tweezers w/3mm diameter platinum disk electrode) CUY650