MTT法和CCK8法检测细胞活性之测试条件比较.pdf

16 5 Vol. 16 No. 5 2007 10 ACTA LASER BIOLOGY SIN ICA O t. 2007 * MTT CCK8 1 1 2 , , ( 1. 华南师范大学物理与电信工程学院, 广东 广州 510006; 2. 西安交通大学生物医学信息工程教育部重点实验 , 生命科学与技术学院, 陕西 西安 710049) : H L60, MTT CCK8 CCK8, 4 h, 490 nm CCK8 OD MTT , : MTT; CCK8; ; : Q233 : A : 10077 146( 2007) An Experim en tal R esearch on D ifferen t D etection Cond ition s betw een M TT and CCK 8 1 1 2 X ION G J ianw en , X IA O H ua , ZHAN G Zhenx i ( 1. S hool of Physi s and T ele omm un i ation Engineering, South Ch ina Norm alUn iversity, Guangzhou 510006, Guangdong, China; 2. The Key Laboratory of B iom edi al Inform ntion Engineering ofM in istry of Edu ation, S hool of L ife S ien e and T e hnology, X i an J iaotong University, X i an 7 10049, Shannx ,i Ch ina) Ab stract: The different dete tion onditions based on HL60 bewt eenMTT and CCK8w ere studied. The best test tmi e is 4 h, the best testw avelength is 490 nm for CCK8. The linearity of CCK8 bewt een OD and ell v iability w as better than those ofMTT. CCK8 was a k ind of ell viab ility assay reagen tw ith a h igher sensitivity and a better reprodu ib ility. Key word s: MTT; CCK8; alibration urves; ell viab ility [ 67] 1983M osm annMTT , , Cell , Counting K it8( CCK8) , CCK8 [ 1] MTT ,